<listing id="kephd"></listing>
<tt id="kephd"></tt>

  • <label id="kephd"></label>
    <meter id="kephd"></meter>

    云南民族大學學報(自然科學版)

    2020, v.29;No.122(04) 317-321

    [打印本頁] [關閉]
    本期目錄(Current Issue) | 過刊瀏覽(Past Issue) | 高級檢索(Advanced Search)

    3,5-二硝基水楊酸法測定液體糖中總糖含量
    Determination of the total sugar content in liquid sugar by 3,5-dinitrosalicylic acid method

    詹夢濤;婁水珠;劉仙花;洪耀強;楊海英;杜剛;
    ZHAN Meng-tao;LOU Shui-zhu;LIU Xian-hua;HONG Yao-qiang;YANG Hai-ying;DU Gang;Key Laboratory of Chemistry in Ethnic Medicinal Resources, State Ethnic Affairs Commission and Ministry of Education;School of Chemistry and Environment, Yunnan Minzu University;

    摘要(Abstract):

    建立3,5-二硝基水楊酸(DNS)測定液體糖中總糖的檢測方法,并對總糖的水解條件和還原糖的檢測條件進行了優化.結果表明,液體糖中總糖的水解條件為:糖液樣品與2 mol/L鹽酸體積比為1∶0.5,50℃水浴加熱5 min;還原糖的檢測條件為:測定波長500 nm,顯色劑DNS用量1.5 mL,沸水浴加熱7 min,顯色時間30 min.標準品葡萄糖在0.1~1.4 mg/mL范圍內呈良好的線性關系,平均加標回收率為99.56%,RSD值為3.16%,相關系數R~2=0.999.精密度、重復性和穩定性試驗結果的相對標準偏差均小于3%,表明該方法可用于液體糖中總糖含量的測定.
    The content of total sugar in liquid sugar was determined by 3,5-dinitrosalicylic acid(DNS) method, the hydrolysis conditions of total sugar and the detection conditions of reducing sugar were optimized. The results showed that the hydrolysis conditions of the total sugar were as follows: the ratio of sugar liquid to 2 mol/L hydrochloric acid was 1∶0.5, with 50 ℃water bath heating 5 min; the detection conditions of reducing sugar in liquid sugar were as follows: the absorption wavelength 500 nm, DNS color developing agent 1.5 mL, the boiling water bath 7 min, and the color developing time 30 min. The glucose in the range of 0.1~1.4 mg/mL(R~2=0.999) was in a good linear relationship, and the recovery rate was 99.56%(RSD=3.16%). The relative standard deviation of repeatability, and the results of precision and stability tests were all less than 3%. The DNS method can be used to determine the total sugar in liquid sugar.

    關鍵詞(KeyWords): 3,5-二硝基水楊酸法;液體糖;總糖;測定
    3,5-dinitrosalicylic acid method;liquid sugar;total sugar content;determination

    Abstract:

    Keywords:

    基金項目(Foundation): 國家自然科學基金(81860624)

    作者(Author): 詹夢濤;婁水珠;劉仙花;洪耀強;楊海英;杜剛;
    ZHAN Meng-tao;LOU Shui-zhu;LIU Xian-hua;HONG Yao-qiang;YANG Hai-ying;DU Gang;Key Laboratory of Chemistry in Ethnic Medicinal Resources, State Ethnic Affairs Commission and Ministry of Education;School of Chemistry and Environment, Yunnan Minzu University;

    Email:

    DOI:

    參考文獻(References):

    擴展功能
    本文信息
    服務與反饋
    本文關鍵詞相關文章
    本文作者相關文章
    中國知網
    分享
    免费资料大全